EsturgeonV1, France, Analysis, bibRecord, 000188

Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa

Identifieur interne : 000188 ( France/Analysis ); précédent : 000187; suivant : 000189

Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa

Auteurs : R. Billard [France] ; J. Cosson [France] ; F. Fierville [France] ; R. Brun [France] ; T. Rouault [France] ; Patrick Williot [France]

Source :

Journal of Applied Ichthyology [ 0175-8659 ] ; 1999-09.

RBID : ISTEX:EAFE77E21C98BF153F6DFDF82C91DA66638A40D8

Abstract

A study of motility was carried out on spermatozoa of the Siberian sturgeon (Acipenser baerii Brandt) reared at the Cemagref experimental research station, St Seurin sur l'Isle, France. About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).

Url:

https://api.istex.fr/document/EAFE77E21C98BF153F6DFDF82C91DA66638A40D8/fulltext/pdf

DOI: 10.1111/j.1439-0426.1999.tb00234.x

Affiliations:

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ISTEX:EAFE77E21C98BF153F6DFDF82C91DA66638A40D8

Le document en format XML

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<front><div type="abstract" xml:lang="en">A study of motility was carried out on spermatozoa of the Siberian sturgeon (Acipenser baerii Brandt) reared at the Cemagref experimental research station, St Seurin sur l'Isle, France. About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).</div>
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Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa

Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri